Today, we will extract this dna using dish soap, salt, water, a coffee filter and alcohol. Squishing breaks cell walls and releases the contents of the cell. Strawberry dna extraction activity institute for molecular. List some practical applications where extracting dna is necessary. Give to students as close to the start of the activity as possible. Add 2 teaspoons of the dna extraction buffer to the bag, zip it up and squeeze it in your hands for 1 minute. Ahead of dealing with strawberry dna extraction lab worksheet answers, remember to are aware that instruction will be our key to an even better another day, along with understanding wont only end once the classes bell rings. Why use strawberries to test your dna extraction kit. Extracted dna can also be used to create dna fingerprints to help diagnose genetic diseases, solve criminal cases, identify victims of disaster and war, and establish paternity or maternity. Dna lab objectives i will extract dna from the fruits that we eat such as strawberry by following dna extraction protocol.
It can easily be seen with the naked eye when collected from thousands of cells. This simple method allows you to extract dna from a strawberry and view it. I also give every student a copy of this free dna extraction lab student sheet also. Squish for a few minutes to completely squash the fruit. Apr 10, 2020 download strawberry dna extraction lab utah state university book pdf free download link or read online here in pdf. These special circumstances make strawberry dna both easy to extract and to see. These instructions guide the development and functioning of all known living organisms and many viruses. Dna is the representation of each and every living organism. Strawberries are an exceptional fruit to use for this lesson because each individual student is able to complete the process by themselves and strawberries yield more dna.
Extract dna from a strawberry national human genome research. Once these membranes are broken apart, the dna is released from the cell. As you gently mash the strawberries, the cells will mix with the dna extracting solution, which is a mixture of soap, salt, and water. This is a simple, effective protocol for spooling dna.
Strawberries are an exceptional fruit to use for this lesson because each individual student is able to complete the process by themselves and strawberries yield more dna than any other fruit i. The purpose of this experiment is to develop the interest, knowledge and skills of our students in the. Dna, or deoxyribonucleic acid, is the basic programming in the cells of all living organisms. Steps i will explain the purpose of each step in the extraction procedure as it relates to the physical and biochemical characteristics of cells. The total number of dna for each fruit had a big variation between strawberries compared to kiwis and bananas with a difference of 5 and 6. Add 10 ml of the dna extraction buffer and mash the strawberry and buffer for about one minute. Each cell in a strawberry contains 8 copies of its genetic information. Dna can be extracted from cells by a simple technique with household chemicals, enabling students to continue reading strawberry dna. Strawberry dna extraction lab every cell in a strawberry contains eight copies of each of its chromosomes. Dissolve the cell membrane and nuclear envelope using detergentsoap current protocols in protein science published by john wiley sons, inc. To extract the dna, each component of the extraction mixture plays a part.
The dish detergent helps dissolve the strawberry cell walls so the dna can be removed. Proficiently build strawberry dna extraction lab worksheet answers focus. You can even see the dna in foods you eat just using tools you have at home. This lab is fun and exciting and one students love. Strawberry dna food science experiments steve spangler. Strawberries are loaded with dna as each cell has eight copies of their chromosomes. But with this strawberry dna extraction science experiment, you can encourage the dna strands to release from their cells and bind together into a format thats visible with the naked eye. The solubility of dna in the rubbing alcohol is also lower when the temperature of the rubbing alcohol is lower. Dna can be extracted from cells by a simple technique with household chemicals, enabling students to.
When an organism has eight copies, called an octoploid, it has a lot more dna per cell than an organism that only has one copy. This experiment show that dna can be extracted in many different ways from the simplest to the most complex way of extracting it. Resea the bag and gently smash for another minute avoid making too many soap bubbles. This starts to break open the cells and release the dna. Apr 07, 2016 dna extraction strawberry shannan muskopf april 7, 2016 in this activity, students crush strawberries and use detergent and salt to break the cell wall so that dna will be released into the solution. Dna extraction c 21 chemistry in the k8 classroom grades 48 2007, omsi orpapaya or pineapple juice. Through this experiment we conclude that each and every. Strawberry dna extraction introduction deoxyribonucleic acid dna is a molecule that encodes genetic instructions. Because of this, they are a perfect fruit to use for a simple strawberry dna lab. I used the alcohol extraction method to isolate dna. Crushing the strawberries breaks open many of the strawberry cells, where the dna is. Strawberry dna extraction lab utah state university pdf. Oct 22, 2010 strawberry dna extraction is a fun experiment that you can perform using everyday materials. Strawberry dna extraction lesson plan this lesson plan is for the extraction of dna from strawberries.
Jun 15, 2018 but with this strawberry dna extraction science experiment, you can encourage the dna strands to release from their cells and bind together into a format thats visible with the naked eye. During a dna extraction, a detergent will cause the cell to pop open, or lyse, so that the dna is released into solution. Choose from 102 different sets of science strawberry dna extraction flashcards on quizlet. Adapted from educationmodulesstrawberryextractioninstructions. Strawberry dna can you extract dna from a strawberry. Read online strawberry dna extraction lab utah state university book pdf free download link book now.
This lesson plan is for the extraction of dna from strawberries. Would you expect the method of dna extraction we used for the strawberry to be the same for human dna. This incredible molecule can be easily seen with the naked eye when. All of them result in students preparing large amounts of dna, enough that everyone can see the dna precipitate out of solution right before their eyes. As a result, strawberries contain large amounts of dna. Because strawberry cells each have eight copies of the genome in every cell. During the experiment why do you think there is dish detergent in the extraction liquid. Strawberries are an exceptional fruit to use for this lesson because each individual student is able to. After the experiment what else can you extract dna from. Use 1 cup of fresh, frozen diluted as directed, or canned juice.
I chose strawberries because they are easy to manipulate and they contain a large genome. Dna is important because it is the chemical code that instructs each cells activity. Though it is much too small to see on its own, it is visible to the human eye en masse. Does anyone have a guess what the soap and tenderizer is for. Similar to the way a builder uses a blueprint to construct a house, cells use dna to construct an organism.
Dna, short for deoxyribonucleic acid, is present in all living cells and is the blueprint that tells each cell how to func on. Doc dna extraction of strawberry and banana jefferson. How to extract strawberry dna with pictures wikihow. Download strawberry dna extraction lab utah state university book pdf free download link or read online here in pdf. Answer any questions from the list made at the beginning of class. Notes and hints keep the isopropyl alcohol very colduse the freezer or ice bucket. Strawberry dna science activity for kids food science.
The salt enables the dna strands to come together, or aggregate. Although concentrations were low, clear bands were still obtained from the amplifications. Each component of the extraction mixture plays a part. Ppt strawberry dna extraction powerpoint presentation. Dna extraction lesson summary in this lesson, students will remove dna from plant cells strawberries and compare the process to dna extraction in an actual biotech lab will discuss in lecture. In order to study our genes, scientists must extract the dna from human tissue. Have students complete the student response worksheet. Strawberry dna extraction all living things have dna. See lab handout strawberry dna extraction questions. This dna extraction lab guides students through learning about the cell membrane and dna all while observing dna they extracted from a strawberry. You can do this experiment with any fruit or vegetable, but strawberries are one of the best fruits to use due to their high number of dna strands per cell. Strawberry % dna % 1 place%several% strawberries %in% asealed%plasc%bag%and%mash% well%with%. Extracting dna is important to scientists, because this greatly aids scientists in finding cure to genetic disorders, new cures to these illnesses in recent years are largely thanks to dna extraction, cures such as bone marrow transplant for sickle cell disease.
My lab includes a student friendly lab sheet, teacher directions and a rubric for summative grading. Add 10 ml dna extraction buffer soapy salty water and squish for a few more minutes. In this lab, students will have a blast extracting dna from a strawberry using common house hold materials to better understand its structure and function. Dna can be extracted from cells by a simple technique with household chemicals, enabling students to see. Learn the simple steps to extract dna from strawberries. In this activity, students will learn the simple method of dna extraction, be able to explain the rationale of each step, and use simple household chemicals to extract dna from a strawberry. What are the three basic steps in any dna extraction. Place a strawberry in a zipclosure bag and remove most of the air before you seal the bag. Strawberry dna extraction everything you need to teach a hands on laboratory investigation on the structure and function of dna. Jun 22, 2018 dna extracting solution mix about 1 cup of dish detergent and 14 cup salt into 4.
Tilt theu and pick up the dna using a plastic coffee stirrer or wooden stick. Dna extraction c 24 chemistry in the k8 classroom grades 48 2007, omsi ongoing assessment why do we need to squish the strawberry. Break the cells open using force 7 dna extraction step 2. Apr 21, 2017 strawberry dna extraction adapted from a lab by c.
Place the strawberry in a zip lock plastic bag and crush it with your fist. Humans have two copies of each chromosome diploid genome. Dna extraction the concentrations of the extracted dna was measured using the nanophotometer,uvvis spectrophotometer implen, munich, germany and the average concentration for 12 samples was 0. Everyone is required to complete and submit the lab sheet upon.
Add 2 teaspoons of the dna extraction liquid into the bag with the strawberry. Strawberries have up to eight copies of each chromosome octoploid genome. The strawberry dna extraction activity, lab protocol and student worksheets were modified and used with permission from julie townsend, parkview middle school, ankeny, ia. Make sure juice contains raw, uncooked fruit juice. Remove proteins that are bound to dna using salt to dissolve them 9 dna extraction step 4. All books are in clear copy here, and all files are secure so dont worry about it.
Dna extraction is important to the study of heredity and to the treatment of many diseases through the creation of gene therapy dna molecules. A chromosome is an organized package of dna found in the nucleus of the cell. Human cells are generally diploid, meaning they have two sets of chromosomes. To keep everyone engaged, i make sure each student got their very own strawberry to smush. For dna extraction, detergent is used to lyse the cell so that dna is released into the solution. I split my students into table groups of 3 and 4 for this lab. Effect of different ratio of the salt mixture to the width of the strawberrys dna. Deoxyribonucleic acid dna is a long molecule that contains the genetic instructions used in the development and functioning of all known living organisms, including the plants we eat.
Dna isolation from strawberries student directions materials per student group strawberries about the volume of a golf ball. Strawberry dna extraction lesson plan primary learning. The dna precipitates out of the solution when the alcohol is added. Strawberries are a great choice for a dna extraction lab because they are octoploid, meaning that they have eight copies of each type of dna chromosome. Strawberries are chosen for this particular experiment because strawberry cell has eight copies of the genome, and it would be easier to extract the dna since it is. Of which becoming stated, we all supply you with a a number of very simple still beneficial articles and web templates manufactured appropriate for every helpful purpose. During the dna extraction, the soap pulls apart the fats lipids and proteins that make up the membranes surrounding the cell and nucleus. The purpose of this experiment is to extract dna from strawberries. Related posts of strawberry dna extraction lab worksheet answers atoms and isotopes worksheet previous to discussing atoms and isotopes worksheet, make sure you are aware that knowledge is definitely our critical for an even better tomorrow, and also learning wont only end once the college bell rings. Kiwis and bananas both together produced the same amount of strawberries alone.
A teaspoons of the dna extraction liquid into the bag with the strawberries. Frozen strawberries should be thawed at room temperature. Athome dna extraction protocol dozens of protocols on the web provide instructions for extracting dna from plants, fruit, wheat germ, etc. Strawberry%dna% 1 place%several%strawberries%in% asealed%plasc%bag%and%mash% well%with%. The complete procedure for the extraction is described using a visual format and there is also a worksheet for students that finish. Dna isolation from strawberries uw genome sciences university. Today, youll just be looking at dna from strawberries, but scientists use fancier.
Learn science strawberry dna extraction with free interactive flashcards. Use a funnel and coffee filters to filter the strawberry juice into a beaker. Using dna from strawberries will help you have a successful dna preparation so you can purify a lot of dna. Many bacterial cells contain only one main chromosome. Extracting dna from strawberries by dania etienne on prezi. Dna extraction lab for strawberries step by step guide. Dna is a double stranded macromolecule composed of nucleotide bases pairing adenine with thymine and guanine with cytosine. Construct an explanation for each step of dna extraction.
54 120 322 1366 782 841 1257 1535 335 253 928 104 1000 1022 705 1436 170 580 1223 1627 88 937 205 421 1108 371 942 27 372 976 768 131 971 694 578 102 571